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smfish probes against bam, egfp, sfgfp rbp9  (Biosearch Technologies Inc)
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Biosearch Technologies Inc smfish probes against bam, egfp, sfgfp rbp9
( A ) Example ovarioles from mCherry RNAi or bam RNAi driven by bam-GAL4 , stained with Hoechst (DNA, blue). White arrows indicate 8 cell egg chambers. Scale bar 50 μm. ( B ) Quantitation of the proportion of ovarioles with one or more 8 cell egg chambers. n = 300 ovarioles for each genotype, from three replicate experiments. p < 0.003, t-test. ( C ) wild type germarium stained for bam <t>mRNA</t> <t>(smFISH,</t> magenta and gray-scale), f-actin (phalloidin, gray-scale), fusome (alpha-spectrin, yellow) and DNA (Hoechst, blue). Images are two z slices from a single stack. A single 2cc is outlined in cyan, and a 4cc is outlined in red. ( Di ) Cartoon depicting the different transgenes used. Staining for GFP protein (green), gfp smFISH (magenta), f-actin (phalloidin, gray-scale) and fusome (alpha-spectrin, yellow) in example germaria from ( ii ) BamFlyFOS ( Sarov et al , 2016 ) ( <t>sfGFP</t> probes), ( iii ) BamPGFP ( eGFP probes). Scale bars 15 μm.
Smfish Probes Against Bam, Egfp, Sfgfp Rbp9, supplied by Biosearch Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
smfish probes against bam, egfp, sfgfp rbp9 - by Bioz Stars, 2026-03
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( A ) Example ovarioles from mCherry RNAi or bam RNAi driven by bam-GAL4 , stained with Hoechst (DNA, blue). White arrows indicate 8 cell egg chambers. Scale bar 50 μm. ( B ) Quantitation of the proportion of ovarioles with one or more 8 cell egg chambers. n = 300 ovarioles for each genotype, from three replicate experiments. p < 0.003, t-test. ( C ) wild type germarium stained for bam mRNA (smFISH, magenta and gray-scale), f-actin (phalloidin, gray-scale), fusome (alpha-spectrin, yellow) and DNA (Hoechst, blue). Images are two z slices from a single stack. A single 2cc is outlined in cyan, and a 4cc is outlined in red. ( Di ) Cartoon depicting the different transgenes used. Staining for GFP protein (green), gfp smFISH (magenta), f-actin (phalloidin, gray-scale) and fusome (alpha-spectrin, yellow) in example germaria from ( ii ) BamFlyFOS ( Sarov et al , 2016 ) ( sfGFP probes), ( iii ) BamPGFP ( eGFP probes). Scale bars 15 μm.

Journal: bioRxiv

Article Title: Destabilisation of bam transcripts terminates the mitotic phase of Drosophila female germline differentiation

doi: 10.1101/2024.07.24.604138

Figure Lengend Snippet: ( A ) Example ovarioles from mCherry RNAi or bam RNAi driven by bam-GAL4 , stained with Hoechst (DNA, blue). White arrows indicate 8 cell egg chambers. Scale bar 50 μm. ( B ) Quantitation of the proportion of ovarioles with one or more 8 cell egg chambers. n = 300 ovarioles for each genotype, from three replicate experiments. p < 0.003, t-test. ( C ) wild type germarium stained for bam mRNA (smFISH, magenta and gray-scale), f-actin (phalloidin, gray-scale), fusome (alpha-spectrin, yellow) and DNA (Hoechst, blue). Images are two z slices from a single stack. A single 2cc is outlined in cyan, and a 4cc is outlined in red. ( Di ) Cartoon depicting the different transgenes used. Staining for GFP protein (green), gfp smFISH (magenta), f-actin (phalloidin, gray-scale) and fusome (alpha-spectrin, yellow) in example germaria from ( ii ) BamFlyFOS ( Sarov et al , 2016 ) ( sfGFP probes), ( iii ) BamPGFP ( eGFP probes). Scale bars 15 μm.

Article Snippet: smFISH probes against bam, egfp, sfgfp and rbp9 were designed with the Stellaris Probe Designer (Biosearch Technologies) (Supplementary Table 1).

Techniques: Staining, Quantitation Assay